The Characteristics of Carotenoid Biosynthesis in Citrus Fruit
نویسندگان
چکیده
To learn how to regulate carotenoid content in citrus fruit, we studied the mechanism of carotenoid accumulation using physiological and genetic methods. Based on differences in the carotenoid profiles of flavedo and juice sacs, citrus species could be roughly divided into three groups: carotenoidpoor, violaxanthin-abundant, and b-cryptoxanthin-abundant groups. A comparison of the gene expression among several citrus species with different carotenoid profiles showed that the difference in carotenoid profiles among citrus species was highly regulated by coordination of the expression for genes related to carotenoid biosynthesis and catabolism. Quantitative trait loci related to carotenoid content were also identified to select progeny with high carotenoid content. Moreover, we showed that the effect of postharvest ethylene on carotenoid accumulation in flavedo varied with temperature. Under an ethylene atmosphere, in flavedo, carotenoid accumulation was enhanced more dramatically than under an ethylene-free atmosphere at 20°C but repressed at 5°C. Discipline: Biotechnology Additional key words: b-cryptoxanthin, DNA marker, ethylene, gene expression, Satsuma mandarin
منابع مشابه
Carotenoid Biosynthesis and their Regulation in Citrus Fruits
Carotenoids are the main pigments responsible for the attractive color of the peel and pulp of citrus fruits and greatly contribute to their nutritional and antioxidant value. Fruits of different Citrus species display a broad array of color singularities and in many cultivars the peel and the pulp also exhibit different color, envisaging specieand tissuespecific regulation of the carotenoid co...
متن کاملComparative transcriptome analyses of a late-maturing mandarin mutant and its original cultivar reveals gene expression profiling associated with citrus fruit maturation
Characteristics of late maturity in fruit are good agronomic traits for extending the harvest period and marketing time. However, underlying molecular basis of the late-maturing mechanism in fruit is largely unknown. In this study, RNA sequencing (RNA-Seq) technology was used to identify differentially expressed genes (DEGs) related to late-maturing characteristics from a late-maturing mutant '...
متن کاملA novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments
Citrus is the first tree crop in terms of fruit production. The colour of Citrus fruit is one of the main quality attributes, caused by the accumulation of carotenoids and their derivative C30 apocarotenoids, mainly β-citraurin (3-hydroxy-β-apo-8'-carotenal), which provide an attractive orange-reddish tint to the peel of oranges and Mandarins. Though carotenoid biosynthesis and its regulation h...
متن کاملThe Effect of Rootstocks on the Sugars, Acids, Carotenoids, Chlorophylls and Ethylene of Clementine Mandarin (Citrus clementina)
Investigations have demonstrated that ratio of sugars to organic acids affects the particular taste of citrus fruit and carotenoids are essential for prevent of the disease. It seems that some rootstocks enhance the ripening of fruits and stimulatethe biosynthesis of sugars and carotenoids. The aim of this study was to evaluate the effect of rootstocks on sugars, organic acids andcarotenoids o...
متن کاملAllelic diversity of phytoene synthase gene influences the transcription level in citrus fruit among a citrus F1 hybrid population
Phytoene synthase (PSY) is one of the key regulatory enzyme on the biosynthesis and accumulation of carotenoid in citrus fruits. The transcriptional diversity of PSY is mainly attributed to the structural variation in promoter region among PSY alleles. In aim to clarify how this transcriptional diversity is regulated among them, PSY alleles responsible for carotenoid biosynthesis in the fruits ...
متن کامل